The BioTecnologie BT team has gained extensive experience in conducting various studies related to Biopesticides over the years. These studies encompass:

• Physical-chemical characterization
• Determination of active substance content
• Measurement of Colony Forming Units (CFU) or direct microscopic counts for viability determination
• Methods validation

They also include studies such as:

• Shelf life studies
  • Cold Stability Testing (0°C) following CIPAC MT 39.3
  • Accelerated Storage Stability per CIPAC MT 46.3
  • Shelf Life evaluations at different temperatures

Additionally, the team conducts tests on treated seeds, including:

• Uniformity of distribution according to CIPAC MT175
• Adhesion to treated seed following CIPAC MT194
• Seed load assessments through CFU determination

Analysis of at least 5 batches, representative of production or pilot-scale, is carried out to determine the content of pure active substances, microbial contaminants, and pathogens.

Manufacturing processes may inadvertently introduce undesired microorganisms, including pathogens and associated toxins. Contaminant and pathogen search and quantification adhere to OECD No. 65 guidelines on microbial contaminant limits for microbial pest control.

The team also specializes in Residue determination, covering plants, plant products, foodstuffs, feedingstuffs, and environmental samples. They can develop analytical methods for residue detection, especially for formulations based on microorganisms. Method validation is routinely performed in matrices resulting from ecotoxicological studies.

Furthermore, the team conducts MIC (Minimum Inhibitory Concentration) determinations for antifungal and antimicrobial substances against yeasts, molds, and bacterial strains. This involves the agar diffusion method using strips test and the broth dilution method following EUCAST or CLSI guidelines. MIC is defined as the lowest concentration of an antimicrobial or antifungal agent that can inhibit visible growth in microorganisms, offering a quantitative estimate of susceptibility.

Quantification of Bacillus thuringiensis δ-endotoxins content is achieved through SDS PAGE and densitometric analysis. This test quantifies these endotoxins in technical powders, broths, and final concentrates from fermentation processes, utilizing electrophoretic separation of soluble proteins based on molecular weights.

Lastly, there are studies assessing the biological compatibility of microbial formulated products with other substances. The evaluation involves measuring Colony Forming Units (CFU) in different contact solutions compared to a control after one or more contact times.