OECD TG 238: Sediment-free Myriophyllum spicatum Toxicity test
Myriophyllum spicatum (aquatic plant in house breeding at B.T.) is cultured in Modified Andrews’ Medium. Fresh lateral branches from pre-culture shortened to 2.5 cm from base are assigned randomly to the test vessels. The test, under static or semi-static conditions, ends after 14 days of exposure to different concentrations of the test substance. Total shoot length and main shoot length, fresh and dry weight are measured.
A toxic reference substance is tested twice a year to check the sensitivity of the organisms.
Range finding test includes from 5 to 7 concentrations of the test substance and 1 untreated control (5 replicates/concentration or control) with one 2,5 cm lateral branch with apical meristem and one end/replicate.
Limit test is carried out with 1 concentration (100 mg/L or at solubility limit) of the test substance and 1 untreated control (10 replicates/concentration and 20 replicates for the control) with one 2,5 cm lateral branch with apical meristem and one end/replicate.
Dose-response test includes from 5 to 7 concentrations of the test substance and
1 untreated control (5 replicates/concentration and 10 replicates for the control) with one 2,5 cm lateral branch with apical meristem and one end/replicate.
Analytical checks, to verify the exposure concentrations in the medium, are performed for static test at time 0 and at 14 days on control and on each test substance sample.
For semi-static test the analysis are carried out at time 0, at 7 days (in spent and fresh solutions) and at 14 days on control and on each test substance sample.
The analysis are performed using a method validated according to SANCO/825/00 rev. 8.1 and SANCO/3029/99 rev. 4 guidance documents.
Specific growth rate and yield for each measured variable: EC50 at 14 days as the main endpoint and as other endpoints: EC10, EC20, LOEC/NOEC.
Study includes GLP management and reporting.
REFERENCES AND GUIDELINES
OECD Guideline fo Testing of Chemicals, No. 238 (26th September 2014) – Sediment-free Myriophyllum spicatum toxicity test.
SANCO/825/00 rev. 8.1 (16/11/2010) – Guidance document on pesticide residue analytical methods.
SANCO/3029/99 rev. 4 (11/07/2000) – Residues: guidance for generating and reporting methods of analysis in support of pre-registration data requirements for Annex II (Part A, section 4) and Annex III (Part A, section 5) of Directive 91/414.
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