Quantification of δ-endotoxins content

The aim of test is the quantification of Bacillus thuringiensis δ-endotoxins in technical powders, broths and final concentrates resulting from fermentation processes. The assay makes use of electrophoretic separation of the soluble proteins based on their moleculare weights. After separation, the relevant protein is quantified by densitometric analysis carried out through specific equipment BIO-RAD Gel Doc 2000TM and its associated software Quantity One.

The Internal method forsees 5 standard concentrations and 1 test item concentration with 3 replicates for each concentration.

of CRY 1 and % of CRY 2

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