{"id":6009,"date":"2022-04-08T10:30:14","date_gmt":"2022-04-08T08:30:14","guid":{"rendered":"https:\/\/www.biotecnologiebt.it\/study\/determination-of-the-minimal-inhibitory-concentration-mic-using-e-test\/"},"modified":"2022-04-28T10:21:53","modified_gmt":"2022-04-28T08:21:53","slug":"determination-of-the-minimal-inhibitory-concentration-mic-using-e-test-2","status":"publish","type":"study","link":"https:\/\/www.biotecnologiebt.it\/it\/study\/determination-of-the-minimal-inhibitory-concentration-mic-using-e-test-2\/","title":{"rendered":"Determination of the Minimal Inhibitory Concentration (MIC) using E-test"},"content":{"rendered":"
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Study<\/span><\/span><\/span><\/div><\/span>[\/vc_column][\/vc_row][vc_row gap=”80″ element_id=”54746″ el_id=”Scientific Contact”][vc_column width=”2\/3″]

Determination of the Minimal Inhibitory Concentration (MIC) using E-test<\/span><\/span><\/span><\/h1><\/span>[vc_column_text animation_delay=”0″ css_animation=”none”]MIC<\/strong>\u00a0is defined as the lowest concentration of an\u00a0antimicrobial<\/strong>\u00a0that will inhibit the visible\u00a0growth<\/strong>\u00a0of a microorganism (fungi and\u00a0yeast<\/strong>) representing a quantitative estimate of the\u00a0susceptibility<\/strong>\u00a0of an organism to a certain antimicrobial agent. E-test consists of a predefined,\u00a0continuous<\/strong>, and exponential gradient of\u00a0antibiotic<\/strong>\u00a0concentrations immobilized along plastic\u00a0test<\/strong>\u00a0strips which, placed on the surface of\u00a0agar plate<\/strong>, can release the agent\u00a0gradually<\/strong>. After the incubation and the microorganism’s growth, the\u00a0point at<\/strong>\u00a0which the drop-shaped\u00a0inhibition<\/strong>\u00a0zone intersects the graded test\u00a0strip<\/strong>\u00a0is defined as the\u00a0MIC<\/strong>\u00a0of the antibiotic.\u00a0 A\u00a0reference<\/strong>\u00a0standard is used for the quality\u00a0check<\/strong>\u00a0of reagents, media and\u00a0inoculum<\/strong>.<\/p>\n

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STUDY DESIGN<\/strong><\/span>
\nThe\u00a0MIC<\/strong>\u00a0value is\u00a0 determined by\u00a0agar diffusion<\/strong>\u00a0procedure using\u00a0E-TEST<\/strong>\u00a0in which specify\u00a0the name<\/strong>\u00a0of substance, the\u00a0n<\/strong>umber<\/strong>\u00a0and the name of\u00a0strains<\/strong>, the number and the name of the\u00a0reference strain<\/strong>. 3 replicates are carried out and MIC value\u00a0is expressed as\u00a0\u00b5g\/mL<\/strong>.<\/p>\n

Study\u00a0includes\u00a0GLP management and reporting<\/strong>\u00a0(if required)<\/p>\n

REFERENCES AND GUIDELINES<\/strong><\/span>
\nAntimicrobial Susceptibility Testing 9302553C<\/strong>\u00a0– en – 2012\/01 and Etest\u00ae for MIC determination of antifungal agents (AB BIODISK)[\/vc_column_text][\/vc_column][vc_column animation_delay=”0″ width=”1\/3″ text_align=”left” element_id=”125942″ css=”.vc_custom_1632387118440{padding-left: px !important;}” crp=”374:i:i,375-767:i:i,768-1023:i:i,1024-1279:i:i,1280-1599:i:i,1600-1919:i:i”][vc_row_inner][vc_column_inner offset=”vc_hidden-lg vc_hidden-md vc_hidden-sm”][vc_empty_space height=”70px”][\/vc_column_inner][\/vc_row_inner][vc_row_inner gap=”40″ element_id=”64139″ el_id=”Business Contact”][vc_column_inner][vc_empty_space height=”15px”]

Study finder<\/span><\/span><\/span><\/h2>[vc_empty_space height=”15px”][vc_column_text animation_delay=”0″][\/vc_column_text][vc_empty_space height=”60px”]

Scientific Contact<\/span><\/span><\/span><\/h3>[vc_empty_space height=”15px”][vc_column_text animation_delay=”0″]\n

Sabrina Mantilacci<\/strong>
\nE-mail:\u00a0mantilacci@biotecnologiebt.it<\/a>
\nPhone: +39 075 895 0045 – Ext. 268[\/vc_column_text][vc_empty_space height=”40px”]

Business Contact<\/span><\/span><\/span><\/h3>[vc_empty_space height=”15px”][vc_column_text animation_delay=”0″]\n

Katy Lazzari<\/strong>
\nE-mail:\u00a0klazzari@biotecnologiebt.it<\/a>
\nPhone: +39 075 895 0045 – Ext. 246<\/p>\n

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