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The ground dwelling insect Aleochara bilineata is recommended in the cases of solid formulations incorporated as such in the soil or formulations to be applied as spray on soil.
Adult beetles, between 1 and 7 days after hatching from Delia antiqua pupae, are exposed to the test substance applied on quartz sand (laboratory test) or on natural standard soil (extended laboratory test) by spraying or by incorporation in order to evaluate the effects on their reproductive capacity. Ten pairs of adult males and females are placed on treated quartz sand. During the following 3 weeks 500 Delia antiqua pupae/week/replicate, are added to the test unit and after 4 weeks from the treatment the adult beetles are removed. The fly pupae are placed in to the hatching units and the effects in terms of reproduction ability over a period from 6 to 8 weeks are assessed recording the number of the newly emerged adult beetles.
Adults of Aleochara bilineata
Range finding is carried out with at least 3 application rates of the test substance and 1 untreated control (at least 2 replicates/treatment or control) with 20 beetles/replicate.
Limit test includes 1 application rate of the test substance, 1 untreated control and 1 toxic reference treatment (4 replicates/treatment or control) with 20 beetles/replicate.
Rate-response test includes at least 5 application rates of the test substance, 1 untreated control and 1 toxic reference treatment (4 replicates/treatment or control) with 20 beetles/replicate.
In order to evaluate the duration of the effects of the test substance and the recovery capacity of the insects it is possible to carry out an extended laboratory test with aged residues of the test substance.
The soil is treated in semi-field conditions and, after an adequate aging time, is moved to laboratory to perform the standard test as previously described.
Reproduction performance: ER50/NOER or EC50/NOEC in terms of mean number of offsprings/beetle,
as option, mortality and behavioural effects are observed.
Study includes GLP management and reporting.
REFERENCES AND GUIDELINES
IOBC/WPRS Guidelines (Mead-Briggs et al., 2000).
ESCORT I Guidance Document (Barret K.L. et al., eds. 1994).
ESCORT II Guidance Document (Candolfi et al., eds. 2001).